| | สโมสร | ผู้จัดการ | ลีก | สนามกีฬา | สถานที่ | ก่อตั้ง |
 | Dynamo Kyiv | Oleksandr Shovkovskyi | Premier Liha | Olimpiysky NSC | Kiev | 1927 |
| Shakhtar Donetsk | Marino Pusic | Premier Liha | Olimpiysky NSC | Donetsk | 1936 |
| FC Metalist 1925 Kharkiv | | Perscha Liga | Metalist Stadium | Kharkiv | 1925 |
| Vorskla Poltava | | Premier Liha | Oleksiy Butovsky Vorskla Stadium | Poltava | 1955 |
| Zorya Luhansk | | Premier Liha | Stadion Avanhard | Luhansk | 1923 |
| Polissya Zhytomyr | Imad Ashur | Premier Liha | Tsentralnyi Stadion Zhytomyr | Zhytomyr | 1959 |
| Tavriya Simferopol | | | RSC Lokomotiv | Simferopol | 1958 |
| Chornomorets Odesa | Oleksandr Babych | Premier Liha | Tsentralnyj | Odessa | 1936 |
| Kryvbas Kryvyi Rih | Yuri Vernydub | Premier Liha | Stadion Shakhty Zhovtneva | Kryvyi Rih | 2001 |
| Kolos Kovalivka | Oleksandr Pozdeev | Premier Liha | Stadion Kovalivka | Hlevakha | 2012 |
| Karpaty Lviv | Vladyslav Lupashko | Premier Liha | Arena Lviv | Lviv | 1963 |
| Rukh Lviv | Vitaliy Ponomaryov | Premier Liha | Skif Stadium | Lviv | 2003 |
| FK Oleksandria | Ruslan Rotan | Premier Liha | Nika | Oleksandria | 1991 |
| LNZ Cherkasy | Andrés Carrasco | Premier Liha | Tsentralnyi Stadion Cherkasy | Cherkasy | 2006 |
| Hoverla Uzhhorod | Vyacheslav Grozny | | Stadion Avanhard | Uzhhorod | 1946 |
| Obolon Kyiv | Sergiy Shyshchenko | Premier Liha | Obolon Arena | Kiev | 1992 |
| Metalurh Zaporizhzhya | Anatoliy Chantsev | Druha Liha | Slavutych Arena | Zaporizhia | 1950 |
| Veres Rivne | Oleh Shandruk | Premier Liha | Avanhard | Rivne | 1957 |
| Volyn Lutsk | | Perscha Liga | Avanhard | Lutsk | 1960 |
| FK Mariupol | | | Illichivets Stadium | Mariupol | 1936 |
| Inhulets Petrove | Vasyl Kobin | Premier Liha | Inhulets Stadium | Petrove | 2013 |
| Metalist Kharkiv | Andriy Anishchenko | Perscha Liga | Metalist Stadium | Kharkiv | 2020 |
| FC Minaj | Zeljko Ljubenovic | Perscha Liga | Avanhard | Mynai | 2015 |
| Livyi Bereg | Vitaliy Pervak | Premier Liha | Stadium Livyi Bereh | Kyiv | 2017 |
| Bukovyna | | Druha Liha | Bukovyna | Chernivtsi | 1958 |
| PFC Sevastopol | | | Sevmorzavod | Sevastopol | 2002 |
| Kobra Kharkiv | | | Helios-Arena | Kharkiv | 2002 |
| SK Poltava | | | Lokomotiv Poltava | Poltava | 2007 |
| Prykarpattia Ivano-Frankivsk | Oleg Rypan | Perscha Liga | Stadion Rukh | Ivano-Frankivsk | 1998 |
| Arsenal Kyiv | | | Lobanovsky Dynamo Stadium | Kiev | 2001 |
| Fk Kramatorsk | | Perscha Liga | Avanhard Kramatorsk | Kramatorsk | 1955 |
| Zirka Kropyvnytskyi | Roman Monarev | | Zirka | Kropyvnytskyi | 1922 |
| Podillya Khmelnytskyi | Serhiy Kovalets | Perscha Liga | Podillya | Khmelnytskyi | 1960 |
| PFC Lviv | | Perscha Liga | Arena Lviv | Lviv | 2006 |
| Stal Altchevsk | Vadym Plotnikov | | Stal | Altchevsk | 1935 |
| Ahrobiznes Volochysk | Oleksandr Chyzhevskyi | Perscha Liga | Yunist Stadium | Volochysk | 2015 |
| FC Sumy | | | Yuvileynyi | Sumy | 2008 |
| Nyva Ternopil | Yuriy Virt | Perscha Liga | Ternopil City Stadium | Ternopil | 1978 |
| Olimpik Donetsk | | Perscha Liga | Olimpik Sports Complex | Donetsk | 2001 |
| FSC Mariupol | Oleg Krasnopyorov | Druha Liha | Azovets Stadium | Mariupol | 2007 |
| UCSA Tarasivka | Dmytro Chyrykal | Druha Liha | Bannikov Stadium | Tarasivka | 2017 |
| Dnipro Dnipropetrovsk | Dmytro Mikhailenko | | Dnipro Arena | Dnipropetrovsk | 1925 |
| Tytan | | | Khimik | Armyansk | 1969 |
| MFK Mykolaiv | | Druha Liha | Tsentralnyi Mykolaiv | Mykolaiv | 1920 |
| Desna Chernihiv | | | Chernihiv Stadium | Chernihiv | 1960 |
| Hirnyk-Sport Komsomolsk | Valeriy Kutsenko | Perscha Liga | Yunist Stadium | Komsomolsk | 1989 |
| Kremin Kremenchuk | Igor Klymovskyi | Perscha Liga | Kremin Stadium | Kremenchuk | 1959 |
| Dinaz Vyshhorod | Oleksandr Ryabokon | Perscha Liga | Dinaz Stadium | Lyutizh | 1999 |
| Viktoriya Sumy | Anatoliy Bezsmertnyi | Perscha Liga | Yuvileynyi | Sumy | 2015 |
| FC Kudrivka-1 | Vasyl Baranov | Perscha Liga | Buzova Arena | Buzova | 1980 |
