| | สโมสร | ผู้จัดการ | ลีก | สนามกีฬา | สถานที่ | ก่อตั้ง |
 | PFK Zvyagel | | | Stadion Avanhard | Zviahel | 2007 |
| Lokomotiv Kyiv | Vadym Lazorenko | Druha Liha | Lokomotyv Stadium | Kyiv | 1919 |
| FC Uzhhorod | Yaromyr Loboda | Druha Liha | Avanhard | Uzhhorod | 2015 |
| FK Mariupol | | | Illichivets Stadium | Mariupol | 1936 |
| Fk Kramatorsk | | | Avanhard Kramatorsk | Kramatorsk | 1955 |
| Kobra Kharkiv | | | Helios-Arena | Kharkiv | 2002 |
| FC Nikopol-NPHU | | | Stadion Elektrometalurh-NFZ | Nikopol | 2009 |
| MFK Mykolaiv | | | Tsentralnyi Mykolaiv | Mykolaiv | 1920 |
| VPK-Ahro Shevchenkivka | | | Kolos Arena | Shevchenkivka | 2010 |
| Alians Lypova Dolyna | | | Yuvileynyi | Lypova Dolyna | 2016 |
| Druzhba Myrivka | | | Kolos Stadium | Myrivka | 1961 |
| Revera 1908 | Yaroslav Matviiv | Druha Liha | Stadion Rukh | Ivano-Frankivsk | 2020 |
| FC Oleksandriya-2 | Viktor Bytsyura | Druha Liha | Nika | Oleksandria | 1991 |
| Metalist 1925-2 Kharkiv | Sergiy Karpenko | Druha Liha | Metalist Stadium | Kharkiv | 1925 |
| Veres-2 Rivne | | | Avanhard | Rivne | 1957 |
| FK Kudrivka | | | Kudrivka Arena | Kudrivka | 1982 |
| Nyva-2 Ternopil | Roman Marych | Druha Liha | Ternopil City Stadium | Ternopil | 1978 |
| Polissya-2 Zhytomyr | Shyl Gautam Kishan | Druha Liha | Tsentralnyi Stadion Zhytomyr | Zhytomyr | 1959 |
| Rukh-2 Lviv | Ivan Fedyk | Druha Liha | Skif Stadium | Lviv | 2003 |
| Vorskla-2 Poltava | Vyacheslav Nivinskyi | Druha Liha | Oleksiy Butovsky Vorskla Stadium | Poltava | 1955 |
| FC Khust | | Perscha Liga | Karpaty Stadium | Khust | 2019 |
| Real Pharma Odesa | | Druha Liha | Spartak Odesa | Odesa | 2000 |
| Kolos-2 Kovalivka | | Druha Liha | Stadion Kovalivka | Hlevakha | 2012 |
| Stal Kamianske | | | Metalurh Dniprodzerzhynsk | Kamianske | 1926 |
| FC Kharkiv | | | Kharkiv Dynamo | Kharkiv | 1998 |
| Stal Altchevsk | Vadym Plotnikov | | Stal | Altchevsk | 1935 |
| Hazovyk Skala Stry | | | Kniazha Arena | Lviv | 1992 |
| Hoverla Uzhhorod | Vyacheslav Grozny | | Stadion Avanhard | Uzhhorod | 1946 |
| FC Odesa | | | Spartak Odesa | Odessa | 1947 |
| Tytan | | | Khimik | Armyansk | 1969 |
| Dynamo 2 Kyiv | | | Dynamo Club Stadium | Kiev | 1992 |
| Krymteplytsia | | | Krymteplytsia | Molodizhne | 1999 |
| Naftovyk-Ukrnafta | | | Naftovyk Stadium | Okhtyrka | 1980 |
| Desna Chernihiv | | | Chernihiv Stadium | Chernihiv | 1960 |
| FC Sumy | | | Yuvileynyi | Sumy | 2008 |
| UkrAgroKom Holokivka | | | Holovkivskyy Stadium | Holokivka | 2008 |
| Peremoha Dnipro | | | Olimpiyski Rezervy | Dnipro | 2017 |
| Kryvbas Kryvy Rih | | | Metalurh | Kryvyi Rih | 1959 |
| FC Ternopil | | | Ternopil City Stadium | Ternopil | 2007 |
| FC Cherkashchyna | | | Tsentralnyi Stadion Cherkasy | Cherkasy | 2010 |
| Metalurh Donetsk | | | Shakhtar | Donetsk | 1996 |
| Dnipro Dnipropetrovsk | Dmytro Mikhailenko | | Dnipro Arena | Dnipropetrovsk | 1925 |
| Zhemchuzhyna Odessa | | | Stadion Spartak | Odessa | 2016 |
| Balkany Zorya | | | Boris Tropants Stadium | Zorya | 2007 |
| Sudnobudivnyk Mykolaiv | | | Tsentralnyi Mykolaiv | Mykolaiv | 2016 |
| FC Kalush | | | Khimik | Kalush | 1947 |
| Krystal Kherson | | | Stadion Krystal | Kherson | 1960 |
| TSK Simferopol | | | RSC Lokomotiv | Simferopol | 2014 |
